Direct and ambient analysis of chemical compounds from surfaces by Desorption Electrospray Ionization
 
Date: 6 Février 2018
 
 

Dans le cadre des "Mardis de la Chimie", Sorbonne Université propose une conférence de André Venter, en anglais, intitulée "Direct and ambient analysis of chemical compounds from surfaces by Desorption Electrospray Ionization". 

Résumé de la conférence:

Analysis and imaging of the surfaces of samples by ambient mass spectrometry is an important new analytical tool that has been used for tumor detection and identification, to study drug actions, catalyst development and other important applications. One of the most widely used ambient ionization mass spectrometry methods is Desorption Electrospray Ionization (DESI). With DESI-MS an electrospray plume is directed at a sample surface to cause both the desorption and ionization of organic molecules, which are then immediately analyzed by mass spectrometry.
Unfortunately, DESI-MS is currently limited to only the analysis of small molecules such as lipids, fatty acids, and other small molecules. Developing the ability to analyze proteins and determine their spatial distribution in tissues and cells would make imaging DESI-MS much more useful for pathology and biotechnology applications.
We have investigated the origin of the mass dependent loss in sensitivity that is experienced with DESI-MS. Based on our findings, I will present some preliminary results on current efforts to improve on the detection of proteins by DESI-MS. These include the addition of ammonium bicarbonate, a very effective signal improving buffer, other additives such as serine, and the implementation of a novel delayed desorption DESI ion source.
 
 
Informations pratiques:
  • date: mardi 06 février 2018 à 16h45 (thé à 16h30)
  • lieu: Amphithéâtre Herpin, Bâtiment Esclangon, Campus Jussieu, 4 place Jussieu, 75005 PARIS. 
  • conférence tout public en libre accès
  • contact: UFR de Chimie, 4, place Jussieu, 75005 Paris; Tél. 01 44 27 29 98; ufrchim@upmc.fr.